For example, v2 and boxer display images using the lower left corner as the (0,0) location while DM and imageJ display images using the upper left corner as the (0,0) location. Also, it is important to understand the co-ordinate system that is used by any given display program. For example, DM automatically ignores the brightest and darkest values in each image (using a "percentage-to-ignore" that the user can control) while v2 and boxer use the full dynamic range (and almost always need manual adjustment of "brightness" and "contrast" before the displayed images look "good" - click the middle mouse button of a 3-button mouse and adjust the sliders labeled "B" and "C"). NOTE: Different display programs will display these images using different parameters and color schemes. However, all the information is available and this is the only way to access that information without using Digital Micrograph to display the file. It will be necessary to familiarize yourself with the organization of this rather user-unfriendly display of that information. In addition, boxer opens another window (labeled "Image View") that is initially blank (solid black in most cases) but that can be used to display boxed out sub-regions of the displayed image. The area displayed in this window can be dragged using the left mouse button and causes appropriate changes to happen in the display window itself. This is somewhat easier when using boxer since you also have a separate window that shows the displayed area as an outline within the boundary of the full image at the right side of the main boxer control window. If the display window is too small to show the full image at the chosen magnification, you can drag the image around using the right mouse button. The size of the display window can be altered boxer from EMAN1by using the mouse pointer and left mouse button to grab and drag an edge or corner of the window to make it larger or smaller. This modifies the magnification of the displayed image but not the size of the display window itself. You can change the apparent magnification of the displayed image by entering values into the "scale" text box to the right of the sliders. Once this window has been opened, the display can be adjusted by using the "B" (brightness) and "C" (contrast) sliders (or by typing values into the text box beside each slider). Images Showing Information Beyond the Nyquist Limitīoth these programs allow the user to modify the display parameters by opening a dialog window using the middle button on a 3-button mouse.Magnification Series from JEOL JEM 3200FS.Magnification Series from JEOL JEM 1010.Scanning Transmission Electron Microscopy (STEM).High Resolution Transmission Electron Microscopy Examples.High Resolution Transmission Electron Microscopy (HRTEM).Energy Filtered Transmission Electron Microscopy (EFTEM).Electron Energy Loss Spectroscopy (EELS).cryo-Transmission Electron Microscopy (cryoTEM).Correlated Light and Electron Microscopy.Using Specific Rappture Interfaces on Karst.Documents for the Leica EM AFS 2 Freeze Substitution Robot.Leica EM AFS-2 Freeze Substitution Device.Inspired by Bill Baxter, who last year made available Matlab functions for reading Spider data files, I have deposited Matlab functions for reading and writing some other. We have been using Matlab for some years for algorithm development and even for 'production' reconstructions. Direct Electron DE-64 Camera Pixel Sizes Next message: 3dem Read MRC, Imagic and DM3 files from Matlab.Gatan UltraScan 4000 Current Pixel Sizes.Gatan UltraScan 4000 Pixel Sizes Before October 2017.Using Drift Correction with the Gatan OneView Camera.The problem may also be with the file itself if the above steps don't work. Check for the following problems with the DM3 file Some applications allow you to update from the program level, and for others you need to download the installer from the Wayne Rasband page and install. The fault may also lie with the ImageJ program, it is possible that DigitalMicrograph Image requires a newer version of the program. behavior of the single - crystal RuO2 ( 110 ), ( 001 ), ( 111 ), ( 101 ), and ( 100 ) surfaces in 0.5 mol / dm3 H2SO4 solution have been studied. " field will set the ImageJ application as the default for handling the DM3 file. If there is no software on the list, simply select "Browse" and manually select the directory where the application is installed. Using the "Change" button we can set the program to handle this type. Information about the file and the program that the file is associated with are available here. It is most convenient to call the context menu with the right mouse button and select Properties. If, despite the installed application, the DM3 file does not start in the ImageJ application, you must create a file association. Create association of ImageJ with DM3 files
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